IDENTIFICATION OF PHENOLIC COMPOUNDS OF CHIMAPHILA UMBELLATA (L.) HERB THROUGH HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

Abstract

The article presents a study devoted to the determination of the qualitative composition of phenolic compounds of Chimaphila umbellata (L.) herb. The of high-performance thin-layer chromatography (HPTLC) method was used to realize the set aim, which is widely used for pharmacopoeial analysis of medicinal plant materials. The dried herb of Chimaphila umbellata (producer of TM “Zelena Apteka”, Zhytomyr) was used for the research. HPLC determinations were performed using a CAMAG instrument consisting of a Linomat 5 semi-automatic sampler, an automatic developing camera 2 (ADC2), a TLC III plate heater, and a TLC visualizer connected to visionCATS 2.1 software. Plates of HPTLC silica gel Si 60 F254, 20 x 10 cm (Merck KGaA, Darmstadt, Germany) were used. Standard samples of chlorogenic acid, caffeic acid, hyperoside, rutin, quercetin, avicularin, isoquercetin and gallic acid were used. The following was used as a mobile phase: ethyl acetate – formic acid – water (68 : 8 : 8), detection of chromatographic zones was carried out using a specific reagent – diphenylboronic acid aminoethyl ether P and macrogol P. Chromatograms were viewed in UV light at wavelengths of 254 nm, 365 nm, and in daylight before and after derivatization. Comparison of the zones in the plant sample with the zones of the standards allowed us to assume the presence of hyperoside (Rf 0.41), avicularin (Rf 0.67), isoquercetin (Rf 0.45), gallic (Rf 0.81), caffeic acids (Rf 0.87) in Chimaphila umbellata herb. Thus, the sufficiently diverse content of phenolic compounds in the herbal raw materials indicates the possibility of standardization of Chimaphila umbellata according to such biologically active compound classes as hydroxycinnamic acids and flavonoids.