STUDY OF THE ANTIATHEROGENIC EFFECT OF THE COMBINED AGENT BASED ON CARROT ROOT EXTRACT AND QUERCETIN ON THE MODEL OF CHOLESTEROL ATHEROSCLEROSIS IN RABBITS
DOI:
https://doi.org/10.32782/health-2024.3.9Keywords:
atherosclerosis, cholesterol, thick carrot root extract, quercetin, antiatherogenic effectAbstract
The aim – study of the antiatherogenic effect of combined tablets based on carrot seed root extract and quercetin on the model of cholesterol atherosclerosis in rabbits. The modeling of experimental atherosclerosis was carried out for 3 months by oral administration of an oil solution of cholesterol at a dose of 0.3 g/kg to rabbits. Combined tablets based on carrot seed root extract thick and quercetin at a dose of 200 mg/kg and comparison drugs tincture “Ravisol”, nicotinic acid tablets, tablets “Vasostat-Zdorovye” were administered in a therapeutic and prophylactic regimen daily once during the reproduction of pathology for 3 months. The condition of the aortic arch, heart muscle and intramural arteries of the heart was assessed histologically with hematoxylin and eosin staining of sections. The presence of lipids was also determined in the aortic arch sections by staining them with Sudan IV. Therapeutic and prophylactic administration of combined tablets based on carrot root extract and quercetin against a cholesterol diet limited the severity of pathological changes in the vascular wall of the aortic arch, which was manifested by the presence of small foci of intimal endothelial proliferation, without lipoidosis in the media. The condition of the majority of intramural arteries of the heart and myocardium of animals improved. The obtained data showed the antiatherogenic effect of combined tablets based on carrot seed root extract and quercetin, according to which the investigated product is superior to Ravisol tincture and nicotinic acid tablets and practically not inferior to Vasostat-Zdorovye tablets. Prolonged administration of exogenous cholesterol caused morphological changes in the state of the vascular wall of the aortic arch, walls of the intramural arteries of the heart, in the inner layers of the myocardium in rabbits, indicating the development of atherosclerosis. Combined tablets with EGMPC and quercetin, when administered therapeutically and prophylactically against a cholesterol diet, have an antiatherogenic effect, reducing the severity of pathological changes in the vascular wall of the aortic arch, intramural arteries of the heart and myocardium of rabbits. In terms of the ability to limit the morphological manifestations of atherosclerosis, combined tablets with EGMPA and quercetin are superior to Ravisol tincture and nicotinic acid tablets and are practically not inferior to Vasostat-Zdorovye tablets.
References
Дячук Д. Д., Мороз Г. З., Гідзинська І. М., Кравченко А. М., Ласиця Т. С., Дзізінська О. О. Профілактика серцево-судинних захворювань: теоретичні засади та практичне впровадження. Київ, 2019. 176 с.
Сіренко Ю. М. Стан проблеми серцево-судинної захворюваності та смертності в Україні. Ліки України. 2022. № 2(258). С. 11–14.
Казимирко В. К., Сілантьєва Т. С., Дубкова А. Г., Крилова А. С., Кутовий В. В., Іваніцька Л. М. Атеросклероз: клітинно-молекулярні механізми розвитку запалення в артеріях. Лiкарська справа. 2022. № 1–2 (1162). С. 25–39.
Ray S. Role of statins in the management of dyslipidaemia. Indian Heart J. 2024. 76 Suppl 1(Suppl 1). Р. 33–37.
Фітотерапія в кардіології : навч. посібник / Ю. І. Корнієвський, О. В. Крайдашенко, М. П. Красько, Н. Ю. Богуславська, В. Г. Корнієвська. Запоріжжя : Вид-во ЗДМУ, 2017. 470 с.
Pavlyuk I., Stadnytska N., Jasicka-Misiak I., Gorka B., Wieczorek P.P., Novikov V. A Study of the Chemical composition and biologic al activity of extracts from Wild Carrot (Daucus carota l.) seeds waste. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2015. Vol. 6 (2). P. 603–611.
Magar R. T., Sohng J. K. A Review on Structure, Modifications and Structure-Activity Relation of Quercetin and Its Derivatives. J Microbiol Biotechnol. 2020. Vol. 30(1). P. 11–20.
Кононенко Т., Чікіткіна В. Експериментальне визначення умовнотерапевтичної дози нового комбінованого гіполіпідемічного засобу на основі кверцетину та моркви посівної коренеплодів екстракту густого на моделі гострої гіперліпідемії. Фітотерапія. Часопис. 2024. № 2. С. 137–146.
Доклінічні дослідження лікарських засобів : метод. рек. / за ред. чл.-кор. НАМН України, акад. О. В. Стефанова. Київ : Авіценна, 2001. C. 263–271.
Maynard R., Downes N., Finney B. Histological techniques: an introduction for beginners in toxicology. Cambridge : Royal Society of Chemistry. 2014. 334 p.
Kiernan J. A. Histological and histochemical methods: theory and practice. 5th ed. Banbury : Scion Publishing. 2015. 571 p.
Layton C., Bancroft J. D., Suvarna S. K. Fixation of tissues. Bancroft’s theory and practice of histological techniques. 8 th ed. St. Louis : Elsevier. 2019. 603 p.
Arya P., Sharma V., Thapliyal S., Sagar R., Singh P. Preclinical models of atherosclerosis: An overview. Iran J Basic Med Sci. 2024. Vol. 27(5). P. 535–542.
Leckey L. C., Garige M., Varatharajalu R., Gong M., Nagata T., Spurney C. F., Lakshman R. M. Quercetin and ethanol attenuate the progression of atherosclerotic plaques with concomitant up regulation of paraoxonase1 (PON1) gene expression and PON1 activity in LDLR-/- mice. Alcohol Clin Exp Res. 2010. Vol. 34(9). P. 1535–1542.
Shakheel M. B., Saliyan T., Satish S., Hedge K. Therapeutic Uses of Daucus carota: A Review. International Journal of Pharma And Chemical Research. 2017. Vol. 3(2). P. 138–143.
