FEATURES OF THE CYTOKINE PROFILE IN THE BLOOD SERUM OF GUINEA PIGS UNDER CONDITIONS OF EXPERIMENTAL ALLERGIC ALVEOLITIS

Abstract

Over the past period, it has been noted that patients with allergic diseases occupy a significant place among visits to medical institutions. In particular, this applies to exogenous alveolitis. Many publications have been published on the role of various physiological, biochemical and immune processes in the pathogenesis of allergic alveolitis. The purpose is to evaluate the role of cytokines TNP–alpha, interleukin–6, and interleukin–10 in the serum of guinea pigs in the formation of allergic alveolitis. Experimental studies on the determination of TNF-alpha, interleukin-6, interleukin-10 were performed in the blood serum of guinea pigs (males) with experimental allergic alveolitis on days 7, 14, 21 of the experiment. The experimental allergic alveolitis was reproduced using the method of Orekhov O.O. The content of these cytokines was determined by enzyme-linked immunosorbent assay (ELISA). A set of reagents for the quantitative enzyme- linked immunosorbent assay of Hi cytokines manufactured by Diacalone (France) was used. Statistical processing of the data was performed using the Student’s method.The role and significance of some cytokines have been determined: TNF-alpha, interleukin-6, interleukin-10 in different days of development of experimental allergic alveolitis in the blood serum of guinea pigs. In particular, a significant increase in the proinflammatory group of cytokines (TNF-α, interleukin-6) was found at all stages of the inflammatory process. At the same time, a slight increase in anti-inflammatory interleukin-10 was recorded in the early period of allergic alveolitis compared with healthy animals and, conversely, a decrease in its content on day fourteen and twenty-first compared with the control group.In our view, the results obtained may indicate cytokine-mediated lung damage in experimental allergic alveolitis, impaired interconnection between nonspecific body protection systems and specific immunity, and probable involvement of other metabolic and immune disorders.