EFFECT OF CELL-FREE CRYOPRESERVED BIOLOGICAL AGENTS ON THE CONTENT OF INDIVIDUAL EICOSANOIDS IN RATS WITH FREUND'S ADJUVANT MODELED ARTHRITIS
DOI:
https://doi.org/10.32782/health-2024.1.4Keywords:
placenta cryoextract, spleen cryoextract, conditioned medium of mesenchymal stem cells, prostaglandins, thromboxane, leukotrienes, autoimmune diseasesAbstract
Rheumatoid arthritis is defined as an inflammatory systemic chronic autoimmune disease that manifests as immune cell infiltration, synovial hyperplasia, pannus formation, and destruction of articular cartilage and bone tissue. Despite its simple definition, inflammation is a complex system of cellular and molecular events, based on a cascade of mediators to obtain specific temporal and spatial responses. Endogenous bioactive lipids are the main mediators of homeostasis, as well as acute and chronic inflammatory processes, participating in the initiation, maintenance, and regression of inflammation. The aim of the work is to characterize the effect of cryoextracts of the placenta (СEP) and spleen (CES), as well as the conditioned medium of mesenchymal stem cells (CM-MSC) on the content of prostaglandin (PG) E2, thromboxane (Tx) B2 and leukotriene (LT) B4 in blood serum on the model of adjuvant arthritis (AA) in shures. Experimental studies were conducted on 42 male rats weighing 200–220 g. AA in rats has all the morphofunctional signs of RA in humans and is accompanied by a typical reaction, the main link of which is T-cell immunity. AA was modeled by subplantar injection of complete Freund's adjuvant (CFA) into the hind right limb at the rate of 0.1 ml per rat. AA treatment was carried out from 14 to 28 days. CEP, CES and CM-MSC were injected intramuscularly with an interval of 2 days (a total of 5 injections), on days 14, 17, 20, 23 and 26, respectively. The development of AA in rats was accompanied by an increase in the level of PGE2 by 34.7% (p<0.01), TxB2 by 72.4% (p<0.001) and LTB4 by 74.7% (p<0.001) compared to the indicators of intact animals, which confirmed the immunoinflammatory nature of the simulated pathology. Against the background of the use of cell-free cryopreserved biological agents, as well as the reference drug diclofenac sodium, a decrease in the level of all studied eicosanoids was noted. The use of cell-free cryopreserved biological agents led to a decrease in the level of the studied eicosanoids in the blood serum of AA rats on the 28th day of the experiment. The most pronounced changes were noted against the background of the introduction of CM-MSC – the level of PGE2 decreased by 24.8% (р<0.05), TxB2 by 30.3% (р<0.01) and LTB4 by 25.6% (р<0.01) relative to the parameters of rats with AA without treatment.
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