FEATURES OF IMMUNOHISTOCHEMICAL DIAGNOSTICS OF CHRONIC ENDOMETRITIS IN WOMEN WITH ENDOMETRIAL HYPERPLASIA WITHOUT ATYPIA
DOI:
https://doi.org/10.32782/health-2025.3.3Keywords:
chronic endometritis, immunohistochemistry, endometrial hyperplasia, inflammation, gynecology, histologyAbstract
Chronic endometritis is a persistent inflammation of the mucous membrane of the uterus of low intensity, characterised by infiltration of the endometrial stroma with plasma cells. For a long time, chronic endometritis has been little studied and rarely considered in clinical practice, since it either proceeds asymptomatically or manifests itself without specific symptoms. Today, despite the fact that scientists have established the unequivocally adverse effect of chronic endometritis on fertility, awareness of this disease among the female population and even among medical professionals in Ukraine remains at a rather low level. The aim of our study was to determine the features of immunohistochemical diagnosis of chronic endometritis in women with endometrial hyperplasia without atypia. Based on the results of the histological study, two study groups were formed: I – endometrium of the proliferation phase (100 samples) and II – endometrium with the phenomenon of hyperplasia without atypia (250 samples). For the verification of chronic endometritis, we used the «gold standard» of diagnosis – immunohistochemical staining with specific antibodies anti-CD-138 (clone EP-201, Rabbit, Vitro, Master Dianostica, Sevilla, Spain) and anti-CD-56 (clone 123C3, Mouse, Vitro, Master Dianostica, Sevilla, Spain).Patients in both groups did not differ significantly in terms of average age (I – 31.99±0.59 years and II – 33.63±0.37). The overall frequency of detection of chronic endometritis is not statistically different in both groups (56% and 58%), as is the frequency of detection of a positive reaction of both markers simultaneously (30% and 27.2%). However, in the group with endometrial hyperplasia, an isolated increase in CD-138 was twice as often recorded (16% and 8%), which can be explained by more frequent and earlier referral for immunohistochemical staining in the presence of clinical signs of hyperplasia. In general, the presence or absence of endometrial hyperplasia without atypia does not affect the incidence of chronic endometritis.
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