АNALYSIS OF THE GLUTATHIONE SYSTEM OF ANTIOXIDANT PROTECTION IN THE LIVER OF RATS WITH EXPERIMENTAL OBESITY AND THE USE OF CARRAGEENAN
DOI:
https://doi.org/10.32782/health-2023.3.6Keywords:
liver, dietary obesity, glutathione system of antioxidant protection, carrageenanAbstract
The aim of our study was to analyze the potential of the glutathione system of antioxidant protection in the liver of rats with experimental obesity and the use of different concentrations of carrageenan. The study was conducted on 40 purebred white male rats weighing 175–185 g. The animals were divided into four groups (10 rats in each group): 1st group – control (intact animals), 2nd – obese animals, 3- 1st – obese animals that received 0.5% carrageenan solution in their diet, 4th – obese animals that consumed 1.0% carrageenan solution. The assessment of the glutathione defense system was carried out by determining the activity of glutathione peroxidase (GP), glutathione-S-transferase (GT), and the concentration of reduced glutathione (GSH). In the liver of rats that consumed a 0.5% carrageenan solution with drinking water, a statistically significantly higher level of GP (by 14.7%, p<0.05), GT (by 20.0%, p<0.05) was found, GSH (by 20.9%, p<0.05), compared to the control values. Indicators also increased in the experimental group that consumed 1.0% carrageenan solution in relation to the intact group of animals, but they were lower in comparison with the 3rd experimental group: GP – by 8.8% (р<0.05), GT – by 6.7% (р<0.05), GSH – by 7.5% (р<0.05). Therefore, the consumption of 0.5% carrageenan in drinking water by rats is accompanied by a more active activity of the antioxidant defense system in the liver tissue than when using 1.0% carrageenan.
References
Nita M., Grzybowski А. The Role of the Reactive Oxygen Species and Oxidative Stress in the Pathomechanism of the AgeRelated Ocular Diseases and Other Pathologies of the Anterior and Posterior Eye Segments in Adults. Oxid Med Cell Longev. 2016. Р. 3164734. DOI: 10.1155/2016/3164734.
G. Glutathione: Biosynthesis and Mechanism of Action. In: Labrou N, Flemetakis E, editors. Glutathione: Biosynthesis, Mechanism of Action and biotechnological implications / Pavarino E. C, Russo A., Galbiatti A. L. S., Almeida W. P., Bertollo E. M. New York: Nova Science, 2013. Ch.1. Р. 1–34.
Oxidative stress and reactive oxygen species: a review of their role in ocular disease / Ung L., Pattamatta U., Carnt N., Wilkinson-Berka J. L., Liew G., White A. J. R. Clin Sci (Lond). 2017. № 131 (24). Р. 2865–2883. doi: 10.1042/ CS20171246.
Мялюк О. П. Особливості перебігу вільнорадикального й енергозабезпечувального окиснення при експериментальному аліментарному ожирінні : дис. … канд. біолог. наук : 03.00.04 ; Тернопільський державний медичний університет імені І. Я. Горбачевського. Тернопіль, 2016. 142 с.
Moyana T. N., Lalonde J. M. Carrageenan-induced intestinal injury in the rat – a model for inflammatory bowel disease. Ann. Clin. Lab. Sci. 1990. Vol. 20. № 6. Р. 420–426.
Резніков О. Загальні етичні принципи експериментів на тваринах. Ендокринологія. 2003. № 8 (1). С. 142–145.
Зінькевич Т., Лісовська В., Стасюк В. Застосування величини р-значення p-value при перевірці статистичних гіпотез. Ринок цінних паперів України. 2012. № 1–2. С. 89–94.
Buchko P., Krynytska I., Marushchak M. Combined effects of κ-carrageenan and monosodium glutamate food additives: effect on free radical oxidation. Romanian Journal of Diabetes Nutrition and Metabolic Diseases. 2021. Vol. 28. № 2. Р. 185–195.
